Individual Species of Diatoms from Natural Populationst

Silicon is an essential element for diatom frustule synthesis and is usually taken up only by dividing cells. With '8Ge, a radioactive analog of Si, the cell cycle marker event of frustule formation was identified for individual species of diatom. The frequency of cells within a population undergoing this division event was estimated, and the cell division rate was calculated. In laboratory cultures, these rates of cell division and those calcplated from changes in cell numbers were similar. By dual labeling with "Ge(OH)4 and NaH 04C03, rates of cell division and photosynthesis were coincidently measured for diatoms both in laboratory cultures and when isolated from natural populations in estuarine, offshore, and polar environments. These techniques permit the coupling between photosynthesis and cell division to be examined in situ for individual species of diatom.